This volume explores the generation and characterization of organoids from different tissues, as these structures allow us to investigate questions about cell commitment and differentiation. Whether pursuing cell development or the genesis or treatment of diseases, organoid research, through the methods and techniques in this collection, aims to provide scientists with a way to regenerate different tissues or organs in their original form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Organoids: Models for Development and Disease serves as an ideal guide for researchers investigating this vital area of study.